Job Title: Technical Research Assistant II
Date Posted: 11-15-2006
Job Location: Massachusetts-Boston
Job Type: Full Time
Education Level: Bachelors Degree
Work Experience: 1+ to 2 Years
Job Shift: First Shift
Yearly Salary:DOE (Depend on Experience)
Company Name: Brigham & Women's Hospital
Company Website:
Contact Information: Brigham & Women's Ho   Brigham & Women's Ho (
Job Description:
Summary: Under the very general supervision of the investigator, performs complex cell culture
and laboratory experiments in the field of Hematology. As experiments proceed, determines
necessary adjustments in design and implements these changes after consultation with

Specific Area of Research: Hematopoiesis - molecular and cellular biology

Principal Duties and Responsibilities:

1. Under the general supervision of the investigator, performs complex laboratory experiments
in the field of Hematologic Malignancies. 

2. As experiments proceed, consults with the investigator and with approval implements
necessary adjustments in experimental design.

3. Records, reviews, and analyzes test results:
a. assesses quality of data;
b. repeats tests as necessary to determine reproducibility of results.

4. Assembles data in useful format for presentation to the investigator: normalization of
results, graphing, etc.

5. Performs routine maintenance on and calibration of laboratory equipment.

6. Responsible for ordering supplies within budgetary limits of the grants.

7. Prepares and determines suitability of solutions, reagents, buffers, and cell culture

8. May assist in the orientation and training of new staff.

9. Will work extensively with mice and is responsible for all aspects of mouse animal
husbandry: establishing crosses, examining litters, weaning juvenile animals, and tagging
animals for identification. Assists in genotyping and breeding of mice to develop models of
human leukemias.

10. Assists in murine bone marrow transplantation of mice, and analysis of leukemic
phenotypes, including flow cytometry, preparation of tissue for histopathologic exam, and
tissue culture experiments. 

Skills and Abilities Required:

Ability to learn and perform a variety of laboratory procedures including: tissue culture,
preparation of DNA and RNA, gel filtration, ion exchange chromatography, electrophoresis, and

Analytical ability and initiative in assessing quality of data and trouble-shooting problems
with experiments.

Visual acuity and manual dexterity

Knowledge of sterile technique

Sound judgment regarding time-planning and laboratory safety

Ability to concentrate

Concern for detail: thoroughness; precision

Ability to withstand visual strain, long periods of standing, and occasional unpleasant

High degree of computer literacy.

Good oral and written communication skills.

Working Conditions:

Laboratory environment; occasionally works with radioactive isotopes, toxic chemicals,
biohazards, and substances emitting unpleasant odors.

Supervisory Responsibility:

May assist in the training and orientation of new staff.  
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