Summary: Under the very general supervision of the investigator, performs complex cell culture
and laboratory experiments in the field of Hematology. As experiments proceed, determines
necessary adjustments in design and implements these changes after consultation with
Specific Area of Research: Hematopoiesis - molecular and cellular biology
Principal Duties and Responsibilities:
1. Under the general supervision of the investigator, performs complex laboratory experiments
in the field of Hematologic Malignancies.
2. As experiments proceed, consults with the investigator and with approval implements
necessary adjustments in experimental design.
3. Records, reviews, and analyzes test results:
a. assesses quality of data;
b. repeats tests as necessary to determine reproducibility of results.
4. Assembles data in useful format for presentation to the investigator: normalization of
results, graphing, etc.
5. Performs routine maintenance on and calibration of laboratory equipment.
6. Responsible for ordering supplies within budgetary limits of the grants.
7. Prepares and determines suitability of solutions, reagents, buffers, and cell culture
8. May assist in the orientation and training of new staff.
9. Will work extensively with mice and is responsible for all aspects of mouse animal
husbandry: establishing crosses, examining litters, weaning juvenile animals, and tagging
animals for identification. Assists in genotyping and breeding of mice to develop models of
10. Assists in murine bone marrow transplantation of mice, and analysis of leukemic
phenotypes, including flow cytometry, preparation of tissue for histopathologic exam, and
tissue culture experiments.
Skills and Abilities Required:
Ability to learn and perform a variety of laboratory procedures including: tissue culture,
preparation of DNA and RNA, gel filtration, ion exchange chromatography, electrophoresis, and
Analytical ability and initiative in assessing quality of data and trouble-shooting problems
Visual acuity and manual dexterity
Knowledge of sterile technique
Sound judgment regarding time-planning and laboratory safety
Ability to concentrate
Concern for detail: thoroughness; precision
Ability to withstand visual strain, long periods of standing, and occasional unpleasant
High degree of computer literacy.
Good oral and written communication skills.
Laboratory environment; occasionally works with radioactive isotopes, toxic chemicals,
biohazards, and substances emitting unpleasant odors.
May assist in the training and orientation of new staff.